Difference between revisions of "Build a core-genome phylogeny"

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# create a new environment
 
conda create -n orthofinder
 
conda create -n orthofinder
 
source activate orthofinder
 
source activate orthofinder
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# install orthofinder
 
conda install orthofinder
 
conda install orthofinder
 +
# install biopython
 +
conda install biopython
 
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Revision as of 12:01, 24 October 2018


Overview

This tutorial describes step by step the reconstruction of a core genome phylogeny for a given clade of bacteria.

Setup

  • clone the necessary repositories
1git clone https://github.com/metagenlab/eutils.git
  • install miniconda
  • create a dedicated environment and install orthofinder
1# create a new environment
2conda create -n orthofinder
3source activate orthofinder
4# install orthofinder
5conda install orthofinder
6# install biopython
7conda install biopython


Get table of genome assemblies

1df -h

Download gbff files

1df -h

Prepare fasta files for orthofinder

Download gbff files