Difference between revisions of "Metagenomics"

From www.wiki.chlamdb.ch
Jump to navigation Jump to search
Line 29: Line 29:
 
<biblio>
 
<biblio>
 
#motus2 pmid=30833550
 
#motus2 pmid=30833550
 +
#lu2019 LU, Jennifer, BREITWIESER, Florian P., THIELEN, Peter, et al. Bracken: estimating species abundance in metagenomics data. PeerJ Computer Science, 2017, vol. 3, p. e104. [https://peerj.com/articles/cs-104/]
 
</biblio>
 
</biblio>

Revision as of 10:17, 5 June 2019

Comparison of species composition fro, shotgun metagnomic data


  • Read classification (kraken-like): needs normalization by genome length to derive relative abundance


Tools

mOTUs2 [1]

  • Phylogenetic profiling based on universal markers
  • Allow to classify genomes for which reference genomes are not available
  • relative abundance


Bracken

links

https://lemmi.ezlab.org


Bibliography

  1. Milanese A, Mende DR, Paoli L, Salazar G, Ruscheweyh HJ, Cuenca M, Hingamp P, Alves R, Costea PI, Coelho LP, Schmidt TSB, Almeida A, Mitchell AL, Finn RD, Huerta-Cepas J, Bork P, Zeller G, and Sunagawa S. Microbial abundance, activity and population genomic profiling with mOTUs2. Nat Commun. 2019 Mar 4;10(1):1014. DOI:10.1038/s41467-019-08844-4 | PubMed ID:30833550 | HubMed [motus2]
  2. LU, Jennifer, BREITWIESER, Florian P., THIELEN, Peter, et al. Bracken: estimating species abundance in metagenomics data. PeerJ Computer Science, 2017, vol. 3, p. e104. [1]

    [lu2019]